› 4h19

X-ray diffraction
1.8Å resolution

Crystal structure of an enolase (mandelate racemase subgroup, target EFI-502087) from agrobacterium tumefaciens, with bound Mg and d-ribonohydroxamate, ordered loop

Released:
DOI: 10.2210/pdb4h19/pdb
PDB entry 4h19 coloured by chain, front view.
PDB entry 4h19 coloured by chain, side view.
PDB entry 4h19 coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Entry authors: Vetting MW, Bouvier JT, Morisco LL, Wasserman SR, Sojitra S, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 372 amino acids
Theoretical weight: 41.12 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7CU39 (Residues: 1-372; Coverage: 100%)
Gene name: Atu4120
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CA 10 x CA
Ligand CL 49 x CL
Ligand MG 16 x MG
Ligand GOL 44 x GOL
Ligand 0YR 16 x 0YR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 178.363Å b: 395.688Å c: 177.802Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.137 0.176
Expression system: Escherichia coli BL21(DE3)

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