› 4h2r

X-ray diffraction
2.47Å resolution

Structure of MHPCO Y270F mutant, 5-hydroxynicotinic acid complex

Released:
DOI: 10.2210/pdb4h2r/pdb
PDB entry 4h2r coloured by chain, front view.
PDB entry 4h2r coloured by chain, side view.
PDB entry 4h2r coloured by chain, top view.
Source organism: Mesorhizobium japonicum MAFF 303099
Entry authors: Kobayashi J, Yoshida H, Mikami B, Hayashi H, Kamitori S, Yagi T

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 379 amino acids
Theoretical weight: 41.78 KDa
Source organism: Mesorhizobium japonicum MAFF 303099
Expression system: Escherichia coli
UniProt:
  • Canonical: Q988D3 (Residues: 1-379; Coverage: 100%)
Gene name: mlr6788
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
3 bound ligands:
Ligand BME 1 x BME
Ligand 5HN 1 x 5HN
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2221
Unit cell:
a: 56.891Å b: 284.553Å c: 125.001Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.261
Expression system: Escherichia coli

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