› 4h4a

X-ray diffraction
2.2Å resolution

Crystal structure of the C-terminal domain of Drosophila melanogaster Zucchini

Released:
DOI: 10.2210/pdb4h4a/pdb
PDB entry 4h4a coloured by chain, front view.
PDB entry 4h4a coloured by chain, side view.
PDB entry 4h4a coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure of the primary piRNA biogenesis factor Zucchini reveals similarity to the bacterial PLD endonuclease Nuc.
Voigt F, Reuter M, Kasaruho A, Schulz EC, Pillai RS, Barabas O
RNA 18 2128-34 (2012)
PMID: 23086923

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194272 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial cardiolipin hydrolase Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.34 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VKD7 (Residues: 89-253; Coverage: 65%)
Gene names: CG12314, zuc
Sequence domains: PLD-like domain
Structure domains: Endonuclease Chain A

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 35.64Å b: 51.28Å c: 40.97Å
α: 90° β: 107.63° γ: 90°
R-values:
R R work R free
0.222 0.218 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Argonaute proteins: functional insights and emerging roles.
Meister G. (2013)
17 more

4 mentions without citation

Crystal structure of the primary piRNA biogenesis factor Zucchini reveals similarity to the bacterial PLD endonuclease Nuc.
Voigt et al. (2012)
3 more