› 4h5q

X-ray diffraction
2.7Å resolution

Crystal Structure of Rift Valley Fever Virus Nucleocapsid Protein Hexamer Bound to Single-stranded DNA

Released:
DOI: 10.2210/pdb4h5q/pdb
PDB entry 4h5q coloured by chain, front view.
PDB entry 4h5q coloured by chain, side view.
PDB entry 4h5q coloured by chain, top view.
Source organism: Rift Valley fever virus
Primary publication:
Phleboviruses encapsidate their genomes by sequestering RNA bases.
Raymond DD, Piper ME, Gerrard SR, Skiniotis G, Smith JL
Proc Natl Acad Sci U S A 109 19208-13 (2012)
PMID: 23129612

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112512 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 245 amino acids
Theoretical weight: 27.37 KDa
Source organism: Rift Valley fever virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: D3K5I7 (Residues: 1-245; Coverage: 100%)
Gene name: N
Sequence domains: Tenuivirus/Phlebovirus nucleocapsid protein
30-mer poly(T) DNA Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.21 KDa
Source organism: Rift Valley fever virus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6422
Unit cell:
a: 108.64Å b: 108.64Å c: 261.29Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.23
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

13 review citations

Current progress in antiviral strategies.
Lou et al. (2014)
12 more

1 mention without citation

Phleboviruses encapsidate their genomes by sequestering RNA bases.
Raymond et al. (2012)