› 4hgw

X-ray diffraction
1.65Å resolution

Crystal structure of S25-2 in complex with a 5,6-dehydro-Kdo disaccharide

Released:
DOI: 10.2210/pdb4hgw/pdb
PDB entry 4hgw coloured by chain, front view.
PDB entry 4hgw coloured by chain, side view.
PDB entry 4hgw coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Exploring the cross-reactivity of S25-2: complex with a 5,6-dehydro-Kdo disaccharide.
Brooks CL, Wimmer K, Kosma P, Müller-Loennies S, Brade L, Brade H, Evans SV
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 2-5 (2013)
PMID: 23295476

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213488 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody Fab fragment, light chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 24.24 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Antibody Fab fragment, heavy chain Chain: B
Molecule details ›
Chain: B
Length: 222 amino acids
Theoretical weight: 24.11 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: KTU, KDO
Ligand KTU 1 x KTU
Ligand KDO 1 x KDO
1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 45.71Å b: 81.3Å c: 131.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.198
Expression system: Mus musculus

Quick links

Citations

3 mentions without citation

Glycosylation of MUC1 influences the binding of a therapeutic antibody by altering the conformational equilibrium of the antigen.
Movahedin et al. (2017)
2 more