› 4hgy

X-ray diffraction
3Å resolution

Structure of the CcbJ Methyltransferase from Streptomyces caelestis

Released:
DOI: 10.2210/pdb4hgy/pdb
PDB entry 4hgy coloured by chain, front view.
PDB entry 4hgy coloured by chain, side view.
PDB entry 4hgy coloured by chain, top view.
Source organism: Streptomyces caelestis
Primary publication:
Structure and possible mechanism of the CcbJ methyltransferase from Streptomyces caelestis.
Bauer J, Ondrovičová G, Najmanová L, Pevala V, Kameník Z, Koštan J, Janata J, Kutejová E
Acta Crystallogr D Biol Crystallogr 70 943-57 (2014)
PMID: 24699640

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 276 amino acids
Theoretical weight: 30.29 KDa
Source organism: Streptomyces caelestis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E9JES0 (Residues: 1-256; Coverage: 100%)
Gene name: HDA41_002763
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 11 x SO4
Ligand EDO 4 x EDO
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 168.18Å b: 245.14Å c: 117.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.225 0.248
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Recent advances in methyltransferase biocatalysis.
Bennett et al. (2017)
1 more