› 4hj2

X-ray diffraction
2.1Å resolution

Crystal Structure Analysis of GSTA1-1 in complex with chlorambucil

Released:
DOI: 10.2210/pdb4hj2/pdb
PDB entry 4hj2 coloured by chain, front view.
PDB entry 4hj2 coloured by chain, side view.
PDB entry 4hj2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The interaction of the chemotherapeutic drug chlorambucil with human glutathione transferase A1-1: kinetic and structural analysis.
Karpusas M, Axarli I, Chiniadis L, Papakyriakou A, Bethanis K, Scopelitou K, Clonis YD, Labrou NE
OpenAccess logo PLoS One 8 e56337 (2013)
PMID: 23460799

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 25.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08263 (Residues: 4-220; Coverage: 98%)
Gene name: GSTA1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand LZ6 2 x LZ6
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: C2
Unit cell:
a: 99Å b: 93.53Å c: 51.18Å
α: 90° β: 93.91° γ: 90°
R-values:
R R work R free
0.215 0.215 0.258
Expression system: Escherichia coli

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Citations

5 review citations

Recent advances in protein engineering and biotechnological applications of glutathione transferases.
Perperopoulou et al. (2018)
4 more

4 mentions without citation

Structural and Biochemical Analyses Reveal the Mechanism of Glutathione S-Transferase Pi 1 Inhibition by the Anti-cancer Compound Piperlongumine.
Harshbarger et al. (2017)
3 more