› 4hkh

X-ray diffraction
1.69Å resolution

Structure of the Hcp1 protein from E. coli EAEC 042 pathovar, mutants N93W-S158W

Released:
DOI: 10.2210/pdb4hkh/pdb
PDB entry 4hkh coloured by chain, front view.
PDB entry 4hkh coloured by chain, side view.
PDB entry 4hkh coloured by chain, top view.
Source organism: Escherichia coli 042
Entry authors: Douzi B, Spinelli S, Derrez E, Blangy S, Brunet YR, Cascales E, Cambillau C

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-505202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type VI secretion protein Chains: A, B, D, E, F, G
Molecule details ›
Chains: A, B, D, E, F, G
Length: 169 amino acids
Theoretical weight: 19.71 KDa
Source organism: Escherichia coli 042
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: D3GUW0 (Residues: 1-163; Coverage: 100%)
Gene name: EC042_4529
Sequence domains: Type VI secretion system effector, Hcp
Structure domains: Hcp1-like

Ligands and Environments

1 bound ligand:
Ligand SO4 11 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 84.21Å b: 145.89Å c: 89.85Å
α: 90° β: 103.42° γ: 90°
R-values:
R R work R free
0.181 0.18 0.196
Expression system: Escherichia coli BL21

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