› 4hod

X-ray diffraction
3.3Å resolution

Crystal structure of LeuT-E290S with bound Cl

Released:
DOI: 10.2210/pdb4hod/pdb
PDB entry 4hod coloured by chain, front view.
PDB entry 4hod coloured by chain, side view.
PDB entry 4hod coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
The chloride binding site of Neurotransmitter Sodium Symporters
Kantcheva AK, Quick M, Shi L, Winther AML, Stolzenberg S, Weinstein H, Javitch JA, Nissen P
Proc. Natl. Acad. Sci. U.S.A. (2013)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chain: A
Molecule details ›
Chain: A
Length: 515 amino acids
Theoretical weight: 57.57 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

4 bound ligands:
Ligand LEU 1 x LEU
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand BOG 5 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3, ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 87.58Å b: 87.48Å c: 80.86Å
α: 90° β: 95° γ: 90°
R-values:
R R work R free
0.268 0.19 0.254
Expression system: Escherichia coli

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