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Volume-conserving trans-cis isomerization pathways in photoactive yellow protein visualized by picosecond X-ray crystallography.

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Jung YO, Lee JH, Kim J, Schmidt M, Moffat K, Srajer V, Ihee H
OpenAccess logo Nat Chem 5 212-20 (2013)
Related entries: 3ve3, 3ve4, 4i38, 4i39, 4i3a, 4i3i, 4i3j

Cited: 77 times
EuropePMC logo PMID: 23422563

Abstract

Trans-to-cis isomerization, the key reaction in photoactive proteins, usually cannot occur through the standard one-bond-flip mechanism. Owing to spatial constraints imposed by a protein environment, isomerization probably proceeds through a volume-conserving mechanism in which highly choreographed atomic motions are expected, the details of which have not yet been observed directly. Here we employ time-resolved X-ray crystallography to visualize structurally the isomerization of the p-coumaric acid chromophore in photoactive yellow protein with a time resolution of 100 ps and a spatial resolution of 1.6 Å. The structure of the earliest intermediate (I(T)) resembles a highly strained transition state in which the torsion angle is located halfway between the trans- and cis-isomers. The reaction trajectory of I(T) bifurcates into two structurally distinct cis intermediates via hula-twist and bicycle-pedal pathways. The bifurcating reaction pathways can be controlled by weakening the hydrogen bond between the chromophore and an adjacent residue through E46Q mutation, which switches off the bicycle-pedal pathway.

Articles - 4hy8 mentioned but not cited (1)

  1. Volume-conserving trans-cis isomerization pathways in photoactive yellow protein visualized by picosecond X-ray crystallography. Jung YO, Lee JH, Kim J, Schmidt M, Moffat K, Srajer V, Ihee H. Nat Chem 5 212-220 (2013)


Reviews citing this publication (13)

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