› 4hyl

X-ray diffraction
1.75Å resolution

The crystal structure of an anti-sigma-factor antagonist from Haliangium ochraceum DSM 14365

Released:
DOI: 10.2210/pdb4hyl/pdb
PDB entry 4hyl coloured by chain, front view.
PDB entry 4hyl coloured by chain, side view.
PDB entry 4hyl coloured by chain, top view.
Source organism: Haliangium ochraceum DSM 14365
Entry authors: Tan K, Chhor G, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112017 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anti-sigma factor antagonist Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 12.95 KDa
Source organism: Haliangium ochraceum DSM 14365
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0LNN2 (Residues: 2-114; Coverage: 99%)
Gene name: Hoch_4443
Sequence domains: STAS domain
Structure domains: STAS domain

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand EDO 5 x EDO
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P32
Unit cell:
a: 59.466Å b: 59.466Å c: 65.599Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.202 0.229
Expression system: Escherichia coli BL21(DE3)

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