› 4i5z

X-ray diffraction
1.8Å resolution

Insulin protein crystallization via langmuir-blodgett

Released:
DOI: 10.2210/pdb4i5z/pdb
PDB entry 4i5z coloured by chain, front view.
PDB entry 4i5z coloured by chain, side view.
PDB entry 4i5z coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
A review of the strategies for obtaining high-quality crystals utilizing nanotechnologies and microgravity.
Pechkova E, Bragazzi N, Bozdaganyan M, Belmonte L, Nicolini C
Crit Rev Eukaryot Gene Expr 24 325-39 (2014)
PMID: 25403962

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134251 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.34 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01317 (Residues: 85-105; Coverage: 26%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01317 (Residues: 25-54; Coverage: 37%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I213
Unit cell:
a: 78.22Å b: 78.22Å c: 78.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.218
Expression system: Escherichia coli

Quick links

Citations

1 review citation

(Nano)biotechnological approaches in the treatment of cervical cancer: integration of engineering and biology.
Xie et al. (2024)
3 other articles

1 mention without citation

Intrinsic property of phenylalanine to trigger protein aggregation and hemolysis has a direct relevance to phenylketonuria.
Anand et al. (2017)