› 4iae

X-ray diffraction
2.05Å resolution

Function and Biology Details

Reaction catalysed: 
GTP = 3',5'-cyclic GMP + diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-571058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme NO-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 21.18 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8YUQ7 (Residues: 1-189; Coverage: 100%)
Gene name: alr2278
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

2 bound ligands:
Ligand 1DX 2 x 1DX
Ligand MLI 3 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P213
Unit cell:
a: 121.913Å b: 121.913Å c: 121.913Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Thirty Years of Saying NO: Sources, Fate, Actions, and Misfortunes of the Endothelium-Derived Vasodilator Mediator.
Vanhoutte et al. (2016)
15 more

4 mentions without citation

Insights into BAY 60-2770 activation and S-nitrosylation-dependent desensitization of soluble guanylyl cyclase via crystal structures of homologous nostoc H-NOX domain complexes.
Kumar et al. (2013)
3 more