› 4idm

X-ray diffraction
2.5Å resolution

Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4idm/pdb
PDB entry 4idm coloured by chain, front view.
PDB entry 4idm coloured by chain, side view.
PDB entry 4idm coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Characterization of the proline-utilization pathway in Mycobacterium tuberculosis through structural and functional studies.
Lagautriere T, Bashiri G, Paterson NG, Berney M, Cook GM, Baker EN
Acta Crystallogr D Biol Crystallogr 70 968-80 (2014)
PMID: 24699642

Function and Biology Details

Reaction catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-129327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-glutamate gamma-semialdehyde dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 563 amino acids
Theoretical weight: 61.56 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: O50443 (Residues: 1-543; Coverage: 100%)
Gene names: Rv1187, rocA
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P622
Unit cell:
a: 162.945Å b: 162.945Å c: 96.227Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.147 0.144 0.207
Expression system: Mycolicibacterium smegmatis

Quick links

Citations

7 review citations

Role of Proline in Pathogen and Host Interactions.
Christgen et al. (2019)
6 more