› 4ihg

X-ray diffraction
2.89Å resolution

Chasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A Production

Released:
DOI: 10.2210/pdb4ihg/pdb
PDB entry 4ihg coloured by chain, front view.
PDB entry 4ihg coloured by chain, side view.
PDB entry 4ihg coloured by chain, top view.
Source organisms:
Primary publication:
Chasing acyl carrier protein through a catalytic cycle of lipid A production.
Masoudi A, Raetz CR, Zhou P, Pemble CW
OpenAccess logo Nature 505 422-6 (2014)
PMID: 24196711

Function and Biology Details

Reaction catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + a UDP-3-O-((3R)-hydroxyacyl)-alpha-D-glucosamine = a UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + a holo-[acyl-carrier-protein]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-539860 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 348 amino acids
Theoretical weight: 36.93 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
Acyl carrier protein Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 80 amino acids
Theoretical weight: 8.87 KDa
Source organism: Escherichia coli str. 'clone D i14'
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand FTT 8 x FTT
Ligand PNS 6 x PNS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 84.18Å b: 89.31Å c: 111.933Å
α: 104.09° β: 92.58° γ: 118.64°
R-values:
R R work R free
0.206 0.204 0.253
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.
Agarwal et al. (2017)
4 more

5 mentions without citation

Fatty acid biosynthesis revisited: structure elucidation and metabolic engineering.
Beld et al. (2015)
4 more