› 4ijg

X-ray diffraction
1.7Å resolution

Crystal structure of monomeric bacteriophytochrome

Released:
DOI: 10.2210/pdb4ijg/pdb
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PDB entry 4ijg coloured by chain, front view.
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PDB entry 4ijg coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
Origins of fluorescence in evolved bacteriophytochromes.
Bhattacharya S, Auldridge ME, Lehtivuori H, Ihalainen JA, Forest KT
J Biol Chem 289 32144-32152 (2014)
PMID: 25253687

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 36.99 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RZA4 (Residues: 1-323; Coverage: 43%)
Gene names: DR_A0050, bphP
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand LBV 1 x LBV
Ligand GOL 2 x GOL
Ligand PO4 1 x PO4
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 94.563Å b: 54.816Å c: 69.77Å
α: 90° β: 91.8° γ: 90°
R-values:
R R work R free
0.206 0.205 0.227
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Near-infrared fluorescent proteins engineered from bacterial phytochromes.
Shcherbakova et al. (2015)
4 more

5 mentions without citation

Phytochromes in Agrobacterium fabrum.
Lamparter et al. (2021)
4 more

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