› 4ik8

X-ray diffraction
1.55Å resolution

High resolution structure of GCaMP3 dimer form 1 at pH 7.5

Released:
DOI: 10.2210/pdb4ik8/pdb
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PDB entry 4ik8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into enhanced calcium indicator GCaMP3 and GCaMPJ to promote further improvement.
Chen Y, Song X, Ye S, Miao L, Zhu Y, Zhang RG, Ji G
Protein Cell 4 299-309 (2013)
PMID: 23549615

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125521 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EF-hand domain-containing protein; Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 448 amino acids
Theoretical weight: 50.54 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: K4DIE3 (Residues: 1-48, 51-59, 60-94, 95-127, 128-147, 279-283, 306-338, 342-354, 361-362, 368-432; Coverage: 58%)
  • Canonical: P42212 (Residues: 2-141, 145-170, 185-187, 201-206, 209-213; Coverage: 74%)
Gene name: GFP
Sequence domains:
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 126.34Å b: 47.352Å c: 66.74Å
α: 90° β: 100.27° γ: 90°
R-values:
R R work R free
0.189 0.179 0.209
Expression system: Escherichia coli

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Citations

8 review citations

Beyond neurovascular coupling, role of astrocytes in the regulation of vascular tone.
Filosa et al. (2016)
7 more

1 mention without citation

Genetically Encoded Fluorescent Biosensor for Rapid Detection of Protein Expression.
Eason et al. (2020)

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