› 4ilg

X-ray diffraction
2.1Å resolution

Crystal structure of Aar2p in complex with the Prp8p RNaseH and Jab1/MPN domains

Released:
DOI: 10.2210/pdb4ilg/pdb
PDB entry 4ilg coloured by chain, front view.
PDB entry 4ilg coloured by chain, side view.
PDB entry 4ilg coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis for dual roles of Aar2p in U5 snRNP assembly.
Weber G, Cristão VF, Santos KF, Jovin SM, Heroven AC, Holton N, Lührmann R, Beggs JD, Wahl MC
Genes Dev 27 525-40 (2013)
PMID: 23442228

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152276 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
A1 cistron-splicing factor AAR2 Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 40.22 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32357 (Residues: 1-355; Coverage: 96%)
Gene names: AAR2, YBL06.06, YBL0611, YBL074C
Sequence domains:
Structure domains:
Pre-mRNA-splicing factor 8 Chain: B
Molecule details ›
Chain: B
Length: 258 amino acids
Theoretical weight: 29.5 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P33334 (Residues: 1836-2090; Coverage: 11%)
Gene names: DBF3, DNA39, PRP8, RNA8, SLT21, USA2, YHR165C
Sequence domains: PRP8 domain IV core
Structure domains:
Pre-mRNA-splicing factor 8 Chain: C
Molecule details ›
Chain: C
Length: 270 amino acids
Theoretical weight: 30.49 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P33334 (Residues: 2147-2413; Coverage: 11%)
Gene names: DBF3, DNA39, PRP8, RNA8, SLT21, USA2, YHR165C
Sequence domains: PROCT (NUC072) domain
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

1 bound ligand:
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 84.768Å b: 63.739Å c: 110.518Å
α: 90° β: 95.39° γ: 90°
R-values:
R R work R free
0.182 0.18 0.228
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Novel regulatory principles of the spliceosomal Brr2 RNA helicase and links to retinal disease in humans.
Mozaffari-Jovin et al. (2014)
2 more

3 mentions without citation

Structural studies of the spliceosome: zooming into the heart of the machine.
Galej et al. (2014)
2 more