› 4ipj

X-ray diffraction
2.15Å resolution

Crystal Structure of R314K N-acetyl Neuraminic Acid Synthase from Neiserria meningitidis with Malate bound

Released:
DOI: 10.2210/pdb4ipj/pdb
PDB entry 4ipj coloured by chain, front view.
PDB entry 4ipj coloured by chain, side view.
PDB entry 4ipj coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Arg314 is essential for catalysis by N-acetyl neuraminic acid synthase from Neisseria meningitidis.
Joseph DD, Jiao W, Parker EJ
Biochemistry 52 2609-19 (2013)
PMID: 23534460

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + N-acetyl-D-mannosamine + H(2)O = phosphate + N-acetylneuraminate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-560698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AFP-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 38.5 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57265 (Residues: 1-349; Coverage: 100%)
Gene names: A6J53_03590, DE8555_0059, NCTC8554_01562, siaC, synC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand LMR 1 x LMR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21212
Unit cell:
a: 58.23Å b: 76.05Å c: 77.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.174 0.242
Expression system: Escherichia coli

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Citations

1 review citation

Flagellin glycosylation with pseudaminic acid in Campylobacter and Helicobacter: prospects for development of novel therapeutics.
Salah Ud-Din et al. (2018)
3 other articles

1 mention without citation

Design, synthesis, and structural elucidation of novel NmeNANAS inhibitors for the treatment of meningococcal infection.
Alwassil et al. (2019)