› 4iqz

X-ray diffraction
2.1Å resolution

The crystal structure of a large insert in RNA polymerase (RpoC) subunit from E. coli

Released:
DOI: 10.2210/pdb4iqz/pdb
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PDB entry 4iqz coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Bhandari V, Sugiman-Marangos SN, Naushad HS, Gupta RS, Junop MS

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit beta' Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 250 amino acids
Theoretical weight: 26.73 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8T7 (Residues: 932-1141; Coverage: 15%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains: RNA polymerase Rpb1, domain 5
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand IOD 56 x IOD
Ligand NA 7 x NA
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 198.06Å b: 65.63Å c: 81.292Å
α: 90° β: 108.92° γ: 90°
R-values:
R R work R free
0.205 0.203 0.24
Expression system: Escherichia coli

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