› 4irp

X-ray diffraction
2.1Å resolution

Crystal structure of catalytic domain of human beta1,4-galactosyltransferase-7 in open conformation with manganses and UDP

Released:
DOI: 10.2210/pdb4irp/pdb
PDB entry 4irp coloured by chain, front view.
PDB entry 4irp coloured by chain, side view.
PDB entry 4irp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of β-1,4-galactosyltransferase 7 enzyme reveal conformational changes and substrate binding.
Tsutsui Y, Ramakrishnan B, Qasba PK
J Biol Chem 288 31963-70 (2013)
PMID: 24052259

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein] = UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-577364 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-1,4-galactosyltransferase 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 29.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBV7 (Residues: 81-327; Coverage: 76%)
Gene names: B4GALT7, UNQ748/PRO1478, XGALT1
Sequence domains:
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

4 bound ligands:
Ligand UDP 2 x UDP
Ligand MN 2 x MN
Ligand ACT 6 x ACT
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P41212
Unit cell:
a: 125.186Å b: 125.186Å c: 87.326Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.275
Expression system: Escherichia coli

Quick links

Citations

10 review citations

A molecular description of cellulose biosynthesis.
McNamara et al. (2015)
9 more

1 mention without citation

Crystal structures of β-1,4-galactosyltransferase 7 enzyme reveal conformational changes and substrate binding.
Tsutsui et al. (2013)