› 4iu4

X-ray diffraction
1.8Å resolution

Crystal structure of Leishmania mexicana arginase in complex with inhibitor BEC

Released:
DOI: 10.2210/pdb4iu4/pdb
PDB entry 4iu4 coloured by chain, front view.
PDB entry 4iu4 coloured by chain, side view.
PDB entry 4iu4 coloured by chain, top view.
Source organism: Leishmania mexicana
Primary publication:
Crystal structure of arginase from Leishmania mexicana and implications for the inhibition of polyamine biosynthesis in parasitic infections.
D'Antonio EL, Ullman B, Roberts SC, Dixit UG, Wilson ME, Hai Y, Christianson DW
Arch Biochem Biophys 535 163-76 (2013)
PMID: 23583962

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 36.03 KDa
Source organism: Leishmania mexicana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6TUJ5 (Residues: 13-329; Coverage: 96%)
Gene name: ARG
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand GOL 1 x GOL
Ligand BME 1 x BME
Ligand S2C 1 x S2C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R3
Unit cell:
a: 88.462Å b: 88.462Å c: 113.963Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.161 0.193
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Arginase Inhibitors: A Rational Approach Over One Century.
Pudlo et al. (2017)
4 more

3 mentions without citation

Polyamines in protozoan pathogens.
Phillips MA. (2018)
2 more