› 4iv8

X-ray diffraction
1.9Å resolution

Crystal structure of N-methyl transferase from Plasmodium knowlesi complexed with S-adenosyl methionine

Released:
DOI: 10.2210/pdb4iv8/pdb
PDB entry 4iv8 coloured by chain, front view.
PDB entry 4iv8 coloured by chain, side view.
PDB entry 4iv8 coloured by chain, top view.
Source organism: Plasmodium knowlesi strain H
Entry authors: Lukk T, Nair SK

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + ethanolamine phosphate = S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphoethanolamine N-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 33.19 KDa
Source organism: Plasmodium knowlesi strain H
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A384KJX3 (Residues: 1-264; Coverage: 100%)
Gene name: PKNH_1258400
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
1 bound ligand:
Ligand BME 2 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21212
Unit cell:
a: 169.71Å b: 96.76Å c: 38.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.233 0.271
Expression system: Escherichia coli BL21(DE3)

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