› 4iwy

X-ray diffraction
2.9Å resolution

SeMet-substituted RimK structure

Released:
DOI: 10.2210/pdb4iwy/pdb
Model geometry
Fit model/data
PDB entry 4iwy coloured by chain, front view.
PDB entry 4iwy coloured by chain, side view.
PDB entry 4iwy coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure and function of Escherichia coli RimK, an ATP-grasp fold, L-glutamyl ligase enzyme.
Zhao G, Jin Z, Wang Y, Allewell NM, Tuchman M, Shi D
Proteins 81 1847-54 (2013)
PMID: 23609986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal protein bS6--L-glutamate ligase Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 35.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C0U4 (Residues: 2-300; Coverage: 100%)
Gene names: JW0836, b0852, rimK
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand GLU 1 x GLU
Ligand SO4 6 x SO4
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P6422
Unit cell:
a: 129.03Å b: 129.03Å c: 171.238Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

C-terminomics: Targeted analysis of natural and posttranslationally modified protein and peptide C-termini.
Tanco et al. (2015)
4 more