› 4j2x

X-ray diffraction
2.85Å resolution

CSL (RBP-Jk) with corepressor KyoT2 bound to DNA

Released:
DOI: 10.2210/pdb4j2x/pdb
PDB entry 4j2x coloured by chain, front view.
PDB entry 4j2x coloured by chain, side view.
PDB entry 4j2x coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure and function of the CSL-KyoT2 corepressor complex: a negative regulator of Notch signaling.
Collins KJ, Yuan Z, Kovall RA
Structure 22 70-81 (2014)
PMID: 24290140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117678 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Recombining binding protein suppressor of hairless Chains: A, C
Molecule details ›
Chains: A, C
Length: 427 amino acids
Theoretical weight: 48.22 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P31266 (Residues: 53-474; Coverage: 80%)
Gene names: Igkjrb1, Igkrsbp, Rbpj, Rbpsuh
Sequence domains:
Structure domains:
Four and a half LIM domains protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 28 amino acids
Theoretical weight: 2.93 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3' Chains: E, G
Molecule details ›
Chains: E, G
Length: 15 nucleotides
Theoretical weight: 4.5 KDa
5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3' Chains: F, H
Molecule details ›
Chains: F, H
Length: 15 nucleotides
Theoretical weight: 4.67 KDa

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 62.157Å b: 97.293Å c: 144.102Å
α: 90° β: 93.17° γ: 90°
R-values:
R R work R free
0.19 0.188 0.231
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Notch signalling in context.
Bray SJ. (2016)
7 more

8 mentions without citation

Biophysics of Notch Signaling.
Sprinzak et al. (2021)
7 more