› 4j7x

X-ray diffraction
2.6Å resolution

Crystal structure of human sepiapterin reductase in complex with sulfasalazine

Released:
DOI: 10.2210/pdb4j7x/pdb
PDB entry 4j7x coloured by chain, front view.
PDB entry 4j7x coloured by chain, side view.
PDB entry 4j7x coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Groenlund Pedersen M, Pojer F, Johnsson K

Function and Biology Details

Reaction catalysed: 
L-erythro-7,8-dihydrobiopterin + NADP(+) = sepiapterin + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152880 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sepiapterin reductase Chains: A, B, F, J
Molecule details ›
Chains: A, B, F, J
Length: 288 amino acids
Theoretical weight: 31.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35270 (Residues: 1-261; Coverage: 100%)
Gene name: SPR
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
4 bound ligands:
Ligand SO4 10 x SO4
Ligand SAS 4 x SAS
Ligand PEG 6 x PEG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P61
Unit cell:
a: 149.194Å b: 149.194Å c: 180.552Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.218 0.242
Expression system: Escherichia coli

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