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Combining crystallography and EPR: crystal and solution structures of the multidomain cochaperone DnaJ.

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Barends TR, Brosi RW, Steinmetz A, Scherer A, Hartmann E, Eschenbach J, Lorenz T, Seidel R, Shoeman RL, Zimmermann S, Bittl R, Schlichting I, Reinstein J
OpenAccess logo Acta Crystallogr D Biol Crystallogr 69 1540-52 (2013)
Cited: 25 times
EuropePMC logo PMID: 23897477

Abstract

Hsp70 chaperones assist in a large variety of protein-folding processes in the cell. Crucial for these activities is the regulation of Hsp70 by Hsp40 cochaperones. DnaJ, the bacterial homologue of Hsp40, stimulates ATP hydrolysis by DnaK (Hsp70) and thus mediates capture of substrate protein, but is also known to possess chaperone activity of its own. The first structure of a complete functional dimeric DnaJ was determined and the mobility of its individual domains in solution was investigated. Crystal structures of the complete molecular cochaperone DnaJ from Thermus thermophilus comprising the J, GF and C-terminal domains and of the J and GF domains alone showed an ordered GF domain interacting with the J domain. Structure-based EPR spin-labelling studies as well as cross-linking results showed the existence of multiple states of DnaJ in solution with different arrangements of the various domains, which has implications for the function of DnaJ.

Reviews - 4j80 mentioned but not cited (4)

  1. Recent advances in the structural and mechanistic aspects of Hsp70 molecular chaperones. Mayer MP, Gierasch LM. J Biol Chem 294 2085-2097 (2019)
  2. Dynamical Structures of Hsp70 and Hsp70-Hsp40 Complexes. Alderson TR, Kim JH, Markley JL. Structure 24 1014-1030 (2016)
  3. The Hsp70-Chaperone Machines in Bacteria. Mayer MP. Front Mol Biosci 8 694012 (2021)
  4. Molecular mechanisms of amyloid formation in living systems. Sinnige T. Chem Sci 13 7080-7097 (2022)

Articles - 4j80 mentioned but not cited (10)

  1. Crucial HSP70 co-chaperone complex unlocks metazoan protein disaggregation. Nillegoda NB, Kirstein J, Szlachcic A, Berynskyy M, Stank A, Stengel F, Arnsburg K, Gao X, Scior A, Aebersold R, Guilbride DL, Wade RC, Morimoto RI, Mayer MP, Bukau B. Nature 524 247-251 (2015)
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  3. Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes. Nillegoda NB, Stank A, Malinverni D, Alberts N, Szlachcic A, Szlachcic A, Barducci A, De Los Rios P, Wade RC, Bukau B. Elife 6 e24560 (2017)
  4. Structural modelling of the DNAJB6 oligomeric chaperone shows a peptide-binding cleft lined with conserved S/T-residues at the dimer interface. Söderberg CAG, Månsson C, Bernfur K, Rutsdottir G, Härmark J, Rajan S, Al-Karadaghi S, Rasmussen M, Höjrup P, Hebert H, Emanuelsson C. Sci Rep 8 5199 (2018)
  5. Combining crystallography and EPR: crystal and solution structures of the multidomain cochaperone DnaJ. Barends TR, Brosi RW, Steinmetz A, Scherer A, Hartmann E, Eschenbach J, Lorenz T, Seidel R, Shoeman RL, Zimmermann S, Bittl R, Schlichting I, Reinstein J. Acta Crystallogr D Biol Crystallogr 69 1540-1552 (2013)
  6. Dissection of structural and functional requirements that underlie the interaction of ERdj3 protein with substrates in the endoplasmic reticulum. Otero JH, Lizák B, Feige MJ, Hendershot LM. J Biol Chem 289 27504-27512 (2014)
  7. The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis. Zhang J, Bai Z, Ouyang M, Xu X, Xiong H, Wang Q, Grimm B, Rochaix JD, Zhang L. EMBO J 40 e106742 (2021)
  8. Evolution and activation mechanism of the flavivirus class II membrane-fusion machinery. Vaney MC, Dellarole M, Duquerroy S, Medits I, Tsouchnikas G, Rouvinski A, England P, Stiasny K, Heinz FX, Rey FA. Nat Commun 13 3718 (2022)
  9. The roles of a flagellar HSP40 ensuring rhythmic beating. Zhu X, Poghosyan E, Rezabkova L, Mehall B, Sakakibara H, Hirono M, Kamiya R, Ishikawa T, Yang P. Mol Biol Cell 30 228-241 (2019)
  10. Nematode CDC-37 and DNJ-13 form complexes and can interact with HSP-90. Schmauder L, Absmeier E, Bepperling A, Barkovits K, Marcus K, Richter K. Sci Rep 11 21346 (2021)


Reviews citing this publication (4)

  1. Structural and functional analysis of the Hsp70/Hsp40 chaperone system. Liu Q, Liang C, Zhou L. Protein Sci 29 378-390 (2020)
  2. X-ray crystallography over the past decade for novel drug discovery - where are we heading next? Zheng H, Handing KB, Zimmerman MD, Shabalin IG, Almo SC, Minor W. Expert Opin Drug Discov 10 975-989 (2015)
  3. Protein folding in vitro and in the cell: From a solitary journey to a team effort. Mecha MF, Hutchinson RB, Lee JH, Cavagnero S. Biophys Chem 287 106821 (2022)
  4. Hsp90, a team player in protein quality control and the stress response in bacteria. Wickramaratne AC, Wickner S, Kravats AN. Microbiol Mol Biol Rev 88 e0017622 (2024)

Articles citing this publication (7)

  1. Structural basis for client recognition and activity of Hsp40 chaperones. Jiang Y, Rossi P, Kalodimos CG. Science 365 1313-1319 (2019)
  2. Sil1, a nucleotide exchange factor for BiP, is not required for antibody assembly or secretion. Ichhaporia VP, Sanford T, Howes J, Marion TN, Hendershot LM. Mol Biol Cell 26 420-429 (2015)
  3. Synergism between a foldase and an unfoldase: reciprocal dependence between the thioredoxin-like activity of DnaJ and the polypeptide-unfolding activity of DnaK. Mattoo RU, Farina Henriquez Cuendet A, Subanna S, Finka A, Priya S, Sharma SK, Goloubinoff P. Front Mol Biosci 1 7 (2014)
  4. Structure of CbpA J-domain bound to the regulatory protein Cbpm explains its specificity and suggests evolutionary link between Cbpm and transcriptional regulators. Sarraf NS, Shi R, McDonald L, Baardsnes J, Zhang L, Cygler M, Ekiel I. PLoS One 9 e100441 (2014)
  5. Hidden information on protein function in censuses of proteome foldedness. Cox D, Ang CS, Nillegoda NB, Reid GE, Hatters DM. Nat Commun 13 1992 (2022)
  6. The self-association equilibrium of DNAJA2 regulates its interaction with unfolded substrate proteins and with Hsc70. Velasco-Carneros L, Cuéllar J, Dublang L, Santiago C, Maréchal JD, Martín-Benito J, Maestro M, Fernández-Higuero JÁ, Orozco N, Moro F, Valpuesta JM, Muga A. Nat Commun 14 5436 (2023)
  7. Overexpression of seagrass DnaJ gene ZjDjB1 enhances the thermotolerance of transgenic arabidopsis thaliana. Chen S, Qiu G. Physiol Mol Biol Plants 27 2043-2055 (2021)


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