› 4j8a

X-ray diffraction
1.26Å resolution

Irradiated-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 145

Released:
DOI: 10.2210/pdb4j8a/pdb
PDB entry 4j8a coloured by chain, front view.
PDB entry 4j8a coloured by chain, side view.
PDB entry 4j8a coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence.
Reddington SC, Rizkallah PJ, Watson PD, Pearson R, Tippmann EM, Jones DD
Angew Chem Int Ed Engl 52 5974-7 (2013)
PMID: 23620472

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 27.89 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand SO4 6 x SO4
Ligand EDO 30 x EDO
Ligand TRS 1 x TRS
2 modified residues:
Ligand CRO 1 x CRO
Ligand HOX 1 x HOX

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 46.5Å b: 58.7Å c: 91.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.13 0.129 0.165
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Genetically encoding phenyl azide chemistry: new uses and ideas for classical biochemistry.
Reddington et al. (2013)
2 more