› 4j8s

X-ray diffraction
1.55Å resolution

Crystal structure of human CNOT1 MIF4G domain in complex with a TTP peptide

Released:
DOI: 10.2210/pdb4j8s/pdb
PDB entry 4j8s coloured by chain, front view.
PDB entry 4j8s coloured by chain, side view.
PDB entry 4j8s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the recruitment of the human CCR4-NOT deadenylase complex by tristetraprolin.
Fabian MR, Frank F, Rouya C, Siddiqui N, Lai WS, Karetnikov A, Blackshear PJ, Nagar B, Sonenberg N
OpenAccess logo Nat Struct Mol Biol 20 735-9 (2013)
PMID: 23644599

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107789 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CCR4-NOT transcription complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 24.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5YKK6 (Residues: 800-1004; Coverage: 8%)
  • Best match: A5YKK6-2 (Residues: 800-999)
Gene names: AD-005, CDC39, CNOT1, KIAA1007, NOT1
Sequence domains: CCR4-NOT transcription complex subunit 1 TTP binding domain
Structure domains: CCR4-NOT transcription complex subunit 1 TTP binding domain
mRNA decay activator protein ZFP36 Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.76 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P26651 (Residues: 312-326; Coverage: 5%)
Gene names: G0S24, NUP475, RNF162A, TIS11A, TTP, ZFP36

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P213
Unit cell:
a: 85.06Å b: 85.06Å c: 85.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.188
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

51 review citations

RNA-binding proteins in immune regulation: a focus on CCCH zinc finger proteins.
Fu et al. (2017)
50 more

13 mentions without citation

Insights into the structure and architecture of the CCR4-NOT complex.
Xu et al. (2014)
12 more