› 4jeb

X-ray diffraction
2.3Å resolution

Crystal structure of an engineered RIDC1 tetramer with four interfacial disulfide bonds and four three-coordinate Zn(II) sites

Released:
DOI: 10.2210/pdb4jeb/pdb
PDB entry 4jeb coloured by chain, front view.
PDB entry 4jeb coloured by chain, side view.
PDB entry 4jeb coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
In vitro and cellular self-assembly of a Zn-binding protein cryptand via templated disulfide bonds.
Medina-Morales A, Perez A, Brodin JD, Tezcan FA
J Am Chem Soc 135 12013-22 (2013)
PMID: 23905754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3112
Unit cell:
a: 54.942Å b: 54.942Å c: 145.658Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.244 0.309
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
6 more

2 mentions without citation

Designed, Helical Protein Nanotubes with Variable Diameters from a Single Building Block.
Brodin et al. (2015)
1 more