› 4jgf

X-ray diffraction
2.5Å resolution

Crystal Structure of the Cataract-Causing P23T gamma D-Crystallin Mutant

Released:
DOI: 10.2210/pdb4jgf/pdb
PDB entry 4jgf coloured by chain, front view.
PDB entry 4jgf coloured by chain, side view.
PDB entry 4jgf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the cataract-causing P23T γD-crystallin mutant.
Ji F, Koharudin LM, Jung J, Gronenborn AM
Proteins 81 1493-8 (2013)
PMID: 23670788

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-crystallin D Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 20.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07320 (Residues: 2-172; Coverage: 98%)
Gene names: CRYG4, CRYGD
Sequence domains: Beta/Gamma crystallin
Structure domains: Crystallins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 37.55Å b: 101.931Å c: 41.371Å
α: 90° β: 96.54° γ: 90°
R-values:
R R work R free
0.233 0.228 0.284
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Lens β-crystallins: the role of deamidation and related modifications in aging and cataract.
Lampi et al. (2014)
1 more

2 mentions without citation

Crystal structure of the cataract-causing P23T γD-crystallin mutant.
Ji et al. (2013)
1 more