› 4jgn

X-ray diffraction
1.86Å resolution

Crystal structure of RNA silencing suppressor p19 with 1nt-5'-overhanging double-helical RNA 20mer pUUG(CUG)5CU

Released:
DOI: 10.2210/pdb4jgn/pdb
PDB entry 4jgn coloured by chain, front view.
PDB entry 4jgn coloured by chain, side view.
PDB entry 4jgn coloured by chain, top view.
Source organism: Tomato bushy stunt virus
Entry authors: Katorcha E, Tamjar J, Popov AN, Malinina L

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159603 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA silencing suppressor p19 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 14.51 KDa
Source organism: Tomato bushy stunt virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69517 (Residues: 27-149; Coverage: 72%)
Gene name: ORF4
Sequence domains: Tombusvirus P19 core protein
Structure domains: RNA silencing suppressor P19
5'-R(P*UP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*U)-3' Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.31 KDa
Source organism: Tomato bushy stunt virus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: R32
Unit cell:
a: 89.362Å b: 89.362Å c: 147.936Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.211
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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