› 4jth

X-ray diffraction
2Å resolution

Crystal structure of F114R/R117Q mutant of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) from Neisseria meningitidis

Released:
DOI: 10.2210/pdb4jth/pdb
PDB entry 4jth coloured by chain, front view.
PDB entry 4jth coloured by chain, side view.
PDB entry 4jth coloured by chain, top view.
Source organism: Neisseria meningitidis MC58
Primary publication:
Examining the role of intersubunit contacts in catalysis by 3-deoxy-d-manno-octulosonate 8-phosphate synthase.
Allison TM, Cochrane FC, Jameson GB, Parker EJ
Biochemistry 52 4676-86 (2013)
PMID: 23746359

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxyphosphooctonate aldolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 30.5 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9JZ55 (Residues: 1-280; Coverage: 100%)
Gene names: NMB1283, kdsA
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand CL 5 x CL
Ligand NA 1 x NA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 82.15Å b: 85.99Å c: 163.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.194
Expression system: Escherichia coli BL21(DE3)

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