› 4jxx

X-ray diffraction
2.3Å resolution

Crystal structure of E coli E. coli glutaminyl-tRNA synthetase bound to tRNA(Gln)(CUG) and ATP from novel cryostabilization conditions

Released:
DOI: 10.2210/pdb4jxx/pdb
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PDB entry 4jxx coloured by chain, side view.
PDB entry 4jxx coloured by chain, top view.
Source organisms:
Primary publication:
Structural and mechanistic basis for enhanced translational efficiency by 2-thiouridine at the tRNA anticodon wobble position.
Rodriguez-Hernandez A, Spears JL, Gaston KW, Limbach PA, Gamper H, Hou YM, Kaiser R, Agris PF, Perona JJ
J Mol Biol 425 3888-906 (2013)
PMID: 23727144

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-289451 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Glutamine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 553 amino acids
Theoretical weight: 63.43 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00962 (Residues: 2-554; Coverage: 100%)
Gene names: JW0666, b0680, glnS
Sequence domains:
Structure domains:
RNA (71-MER) Chain: B
Molecule details ›
Chain: B
Length: 75 nucleotides
Theoretical weight: 24.06 KDa
Source organism: Escherichia coli
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

2 bound ligands:
Ligand ATP 1 x ATP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: C2221
Unit cell:
a: 93.66Å b: 234.28Å c: 113.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.258
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

13 review citations

Celebrating wobble decoding: Half a century and still much is new.
Agris et al. (2018)
12 more

5 mentions without citation

Personalized Medicine in Mitochondrial Health and Disease: Molecular Basis of Therapeutic Approaches Based on Nutritional Supplements and Their Analogs.
Tragni et al. (2022)
4 more

PDB-REDO

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