› 4k17

X-ray diffraction
2.9Å resolution

Crystal Structure of mouse CARMIL residues 1-668

Released:
DOI: 10.2210/pdb4k17/pdb
PDB entry 4k17 coloured by chain, front view.
PDB entry 4k17 coloured by chain, side view.
PDB entry 4k17 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
CARMIL leading edge localization depends on a non-canonical PH domain and dimerization.
Zwolak A, Yang C, Feeser EA, Ostap EM, Svitkina T, Dominguez R
OpenAccess logo Nat Commun 4 2523 (2013)
PMID: 24071777

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
F-actin-uncapping protein LRRC16A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 669 amino acids
Theoretical weight: 74.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6EDY6 (Residues: 1-668; Coverage: 49%)
Gene names: Carmil, Carmil1, Lrrc16, Lrrc16a
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand OHB 1 x OHB
Ligand CL 1 x CL
Ligand ABU 1 x ABU
1 modified residue:
Ligand MSE 76 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P1
Unit cell:
a: 57.168Å b: 67.986Å c: 212.151Å
α: 92.73° β: 96.95° γ: 110.15°
R-values:
R R work R free
0.215 0.215 0.259
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Steering cell migration: lamellipodium dynamics and the regulation of directional persistence.
Krause et al. (2014)
2 more

6 mentions without citation

Genomic analysis of 220 CTCLs identifies a novel recurrent gain-of-function alteration in RLTPR (p.Q575E).
Park et al. (2017)
5 more