› 4k1s

X-ray diffraction
1.96Å resolution

Gly-Ser-SplB protease from Staphylococcus aureus at 1.96 A resolution

Released:
DOI: 10.2210/pdb4k1s/pdb
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Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Primary publication:
Staphylococcal SplB serine protease utilizes a novel molecular mechanism of activation.
Pustelny K, Zdzalik M, Stach N, Stec-Niemczyk J, Cichon P, Czarna A, Popowicz G, Mak P, Drag M, Salvesen GS, Wladyka B, Potempa J, Dubin A, Dubin G
J Biol Chem 289 15544-53 (2014)
PMID: 24713703

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease SplB Chains: A, B
Molecule details ›
Chains: A, B
Length: 206 amino acids
Theoretical weight: 22.55 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FXC3 (Residues: 37-240; Coverage: 100%)
Gene names: SAOUHSC_01941, splB
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P41212
Unit cell:
a: 117.9Å b: 117.9Å c: 73.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.247
Expression system: Escherichia coli

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Citations

10 citation in other articles

Transcript Profiling of MRSA Biofilms Treated with a Halogenated Phenazine Eradicating Agent: A Platform for Defining Cellular Targets and Pathways Critical to Biofilm Survival.
Abouelhassan et al. (2018)
9 more

2 mentions without citation

Staphylococcal SplB serine protease utilizes a novel molecular mechanism of activation.
Pustelny et al. (2014)
1 more

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