› 4k5a

X-ray diffraction
1.5Å resolution

Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W

Released:
DOI: 10.2210/pdb4k5a/pdb
PDB entry 4k5a coloured by chain, front view.
PDB entry 4k5a coloured by chain, side view.
PDB entry 4k5a coloured by chain, top view.
Source organisms:
Primary publication:
Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W.
Schilling J, Schöppe J, Sauer E, Plückthun A
J Mol Biol 426 2346-62 (2014)
PMID: 24747052

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164659 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bcl-2-like protein 2 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.19 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1RMX3 (Residues: 2-171; Coverage: 88%)
Gene name: BCL2L2
Sequence domains:
Designed Ankyrin Repeat Protein 013_D12 Chain: B
Molecule details ›
Chain: B
Length: 169 amino acids
Theoretical weight: 18.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 110.7Å b: 42.6Å c: 76.7Å
α: 90° β: 110.8° γ: 90°
R-values:
R R work R free
0.158 0.156 0.19
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
2 more

2 mentions without citation

Computationally designed high specificity inhibitors delineate the roles of BCL2 family proteins in cancer.
Berger et al. (2016)
1 more