› 4k8j

X-ray diffraction
2Å resolution

Crystal Structure of Staphylococcal nuclease mutant V23L/V66I

Released:
DOI: 10.2210/pdb4k8j/pdb
PDB entry 4k8j coloured by chain, front view.
PDB entry 4k8j coloured by chain, side view.
PDB entry 4k8j coloured by chain, top view.
Source organism: Staphylococcus aureus
Entry authors: Sanders JM, Gill E, Roeser JR, Janowska K, Sakon J, Stites WE

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.44 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00644 (Residues: 89-223; Coverage: 66%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41
Unit cell:
a: 48.2Å b: 48.2Å c: 63.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.251
Expression system: Escherichia coli

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