Function and Biology Details
Reaction catalysed:
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Botulinum neurotoxin type B and Synaptotagmin-2 (preferred)
PDBe Complex ID:
PDB-CPX-536174 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Botulinum neurotoxin B heavy chain
Molecule details ›
Chains: A, B
Length: 459 amino acids
Theoretical weight: 54.92 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 459 amino acids
Theoretical weight: 54.92 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
- Canonical:
P10844 (Residues: 857-1291; Coverage: 34%)
Sequence domains:
- Clostridium neurotoxin, N-terminal receptor binding
- Clostridium neurotoxin, C-terminal receptor binding
Synaptotagmin-2
Molecule details ›
Chains: C, D
Length: 68 amino acids
Theoretical weight: 7.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
Length: 68 amino acids
Theoretical weight: 7.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
- Canonical: P46097 (Residues: 8-61; Coverage: 13%)
Ligands and Environments
No modified residues
Experiments and Validation Details
X-ray source:
BESSY BEAMLINE 14.1
Spacegroup:
P21
Expression system: Escherichia coli
