› 4kig

X-ray diffraction
2.4Å resolution

Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with 4-hydroxyphenylpyruvic acid

Released:
DOI: 10.2210/pdb4kig/pdb
PDB entry 4kig coloured by chain, front view.
PDB entry 4kig coloured by chain, side view.
PDB entry 4kig coloured by chain, top view.
Source organism: Streptomyces hygroscopicus
Primary publication:
Structure and mechanism of a nonhaem-iron SAM-dependent C-methyltransferase and its engineering to a hydratase and an O-methyltransferase.
Zou XW, Liu YC, Hsu NS, Huang CJ, Lyu SY, Chan HC, Chang CY, Yeh HW, Lin KH, Wu CJ, Tsai MD, Li TL
Acta Crystallogr D Biol Crystallogr 70 1549-60 (2014)
PMID: 24914966

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 3-phenylpyruvate = S-adenosyl-L-homocysteine + (3S)-2-oxo-3-phenylbutanoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179134 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylpyruvate C(3)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 357 amino acids
Theoretical weight: 39.4 KDa
Source organism: Streptomyces hygroscopicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q643C8 (Residues: 1-337; Coverage: 100%)
Gene name: mppJ
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ENO 2 x ENO
Ligand FE 2 x FE
Ligand 34H 2 x 34H
Ligand CA 9 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P212121
Unit cell:
a: 59.485Å b: 81.145Å c: 137.422Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.168 0.248
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.
Keatinge-Clay AT. (2017)
4 more