4kkj › Citations

Structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin.

Furger E, Frei DC, Schibli R, Fischer E, Prota AE
J Biol Chem 288 25466-25476 (2013)
Cited: 23 times
EuropePMC logo PMID: 23846701

Abstract

Cobalamin (Cbl; vitamin B12) is an essential micronutrient synthesized only by bacteria. Mammals have developed a sophisticated uptake system to capture the vitamin from the diet. Cbl transport is mediated by three transport proteins: transcobalamin, intrinsic factor, and haptocorrin (HC). All three proteins have a similar overall structure but a different selectivity for corrinoids. Here, we present the crystal structures of human HC in complex with cyanocobalamin and cobinamide at 2.35 and 3.0 Å resolution, respectively. The structures reveal that many of the interactions with the corrin ring are conserved among the human Cbl transporters. However, the non-conserved residues Asn-120, Arg-357, and Asn-373 form distinct interactions allowing for stabilization of corrinoids other than Cbl. A central binding motif forms interactions with the e- and f-side chains of the corrin ring and is conserved in corrinoid-binding proteins of other species. In addition, the α- and β-domains of HC form several unique interdomain contacts and have a higher shape complementarity than those of intrinsic factor and transcobalamin. The stabilization of ligands by all of these interactions is reflected in higher melting temperatures of the protein-ligand complexes. Our structural analysis offers fundamental insights into the unique binding behavior of HC and completes the picture of Cbl interaction with its three transport proteins.

Articles - 4kkj mentioned but not cited (1)

  1. Structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin. Furger E, Frei DC, Schibli R, Fischer E, Prota AE. J Biol Chem 288 25466-25476 (2013)


Reviews citing this publication (5)

  1. Vitamin B-12 and Perinatal Health. Finkelstein JL, Layden AJ, Stover PJ. Adv Nutr 6 552-563 (2015)
  2. Stable mammalian producer cell lines for structural biology. Büssow K. Curr Opin Struct Biol 32 81-90 (2015)
  3. Recent trends in the development of vitamin B12 derivatives for medicinal applications. Zelder F. Chem Commun (Camb) 51 14004-14017 (2015)
  4. Antivitamins for Medicinal Applications. Zelder F, Sonnay M, Prieto L. Chembiochem 16 1264-1278 (2015)
  5. Bioprocess Strategies for Vitamin B12 Production by Microbial Fermentation and Its Market Applications. Calvillo Á, Pellicer T, Carnicer M, Planas A. Bioengineering (Basel) 9 365 (2022)

Articles citing this publication (17)

  1. Functional and structural characterization of an ECF-type ABC transporter for vitamin B12. Santos JA, Rempel S, Mous ST, Pereira CT, Ter Beek J, de Gier JW, Guskov A, Slotboom DJ. Elife 7 e35828 (2018)
  2. Letter Cryo-EM structure of human lysosomal cobalamin exporter ABCD4. Xu D, Feng Z, Hou WT, Jiang YL, Wang L, Sun L, Zhou CZ, Chen Y. Cell Res 29 1039-1041 (2019)
  3. Antivitamins B12--A Structure- and Reactivity-Based Concept. Kräutler B. Chemistry 21 11280-11287 (2015)
  4. Structural basis of transcobalamin recognition by human CD320 receptor. Alam A, Woo JS, Schmitz J, Prinz B, Root K, Chen F, Bloch JS, Zenobi R, Locher KP. Nat Commun 7 12100 (2016)
  5. Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM. Rempel S, Colucci E, de Gier JW, Guskov A, Slotboom DJ. Nat Commun 9 3038 (2018)
  6. Conformational Change of a Tryptophan Residue in BtuF Facilitates Binding and Transport of Cobinamide by the Vitamin B12 Transporter BtuCD-F. Mireku SA, Ruetz M, Zhou T, Korkhov VM, Kräutler B, Locher KP. Sci Rep 7 41575 (2017)
  7. Heme Uptake in Lactobacillus sakei Evidenced by a New Energy Coupling Factor (ECF)-Like Transport System. Verplaetse E, André-Leroux G, Duhutrel P, Coeuret G, Chaillou S, Nielsen-Leroux C, Champomier-Vergès MC. Appl Environ Microbiol 86 e02847-19 (2020)
  8. Functional and phylogenetic characterization of noncanonical vitamin B12-binding proteins in zebrafish suggests involvement in cobalamin transport. Benoit CR, Stanton AE, Tartanian AC, Motzer AR, McGaughey DM, Bond SR, Brody LC. J Biol Chem 293 17606-17621 (2018)
  9. Basal Gnathostomes provide unique insights into the evolution of vitamin B12 binders. Lopes-Marques M, Ruivo R, Delgado I, Wilson JM, Aluru N, Castro LF. Genome Biol Evol 7 457-464 (2014)
  10. Organometallic B12-DNA conjugate: synthesis, structure analysis, and studies of binding to human B12-transporter proteins. Hunger M, Mutti E, Rieder A, Enders B, Nexo E, Kräutler B. Chemistry 20 13103-13107 (2014)
  11. Expression of TCN1 in Blood is Negatively Associated with Verbal Declarative Memory Performance. Akkouh IA, Ueland T, Andreassen OA, Brattbakk HR, Steen VM, Hughes T, Djurovic S. Sci Rep 8 12654 (2018)
  12. Photodissociation of ethylphenylcobalamin antivitamin B12. Lodowski P, Ciura K, Toda MJ, Jaworska M, Kozlowski PM. Phys Chem Chem Phys 19 30310-30315 (2017)
  13. Structure of the human transcobalamin beta domain in four distinct states. Bloch JS, Ruetz M, Kräutler B, Locher KP. PLoS One 12 e0184932 (2017)
  14. Vitamin B12 Phosphate Conjugation and Its Effect on Binding to the Human B12 -Binding Proteins Intrinsic Factor and Haptocorrin. Ó Proinsias K, Ociepa M, Pluta K, Chromiński M, Nexo E, Gryko D. Chemistry 22 8282-8289 (2016)
  15. 5'-Vitamin B12 derivatives suitable for bioconjugation via the amide bond. Jackowska A, Chromiński M, Giedyk M, Gryko D. Org Biomol Chem 16 936-943 (2018)
  16. Organometallic DNA-B12 Conjugates as Potential Oligonucleotide Vectors: Synthesis and Structural and Binding Studies with Human Cobalamin-Transport Proteins. Mutti E, Hunger M, Fedosov S, Nexo E, Kräutler B. Chembiochem 18 2280-2291 (2017)
  17. Loss of the Vitamin B-12 Transport Protein Tcn2 Results in Maternally Inherited Growth and Developmental Defects in Zebrafish. Benoit CR, Walsh DJ, Mekerishvili L, Houerbi N, Stanton AE, McGaughey DM, Brody LC. J Nutr 151 2522-2532 (2021)


Quick links