4kky

X-ray diffraction
2Å resolution

Crystal structure of N-(1-Pyrene)acetamide labeled P450cam in substrate bound form.

Released:
Model geometry
Fit model/data
Source organism: Pseudomonas putida
Entry authors: Zhang M, Goodin DB

Function and Biology Details

Reaction catalysed:
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: X
Molecule details ›
Chain: X
Length: 413 amino acids
Theoretical weight: 46.4 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 2-414; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 35.95Å b: 101.99Å c: 57.35Å
α: 90° β: 101.73° γ: 90°
R-values:
R R work R free
0.173 0.17 0.225
Expression system: Escherichia coli