› 4kl7

X-ray diffraction
1.45Å resolution

Crystal structure of the catalytic domain of RpfB from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4kl7/pdb
PDB entry 4kl7 coloured by chain, front view.
PDB entry 4kl7 coloured by chain, side view.
PDB entry 4kl7 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Carbohydrate recognition by RpfB from Mycobacterium tuberculosis unveiled by crystallographic and molecular dynamics analyses.
Squeglia F, Romano M, Ruggiero A, Vitagliano L, De Simone A, Berisio R
Biophys J 104 2530-9 (2013)
PMID: 23746526

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Resuscitation-promoting factor RpfB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 80 amino acids
Theoretical weight: 8.53 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WG29 (Residues: 283-362; Coverage: 24%)
Gene names: MTC1237.26, Rv1009, rpfB
Sequence domains: Transglycosylase-like domain
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 42.427Å b: 51.595Å c: 66.594Å
α: 90° β: 104.06° γ: 90°
R-values:
R R work R free
0.147 0.145 0.192
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Lytic transglycosylases: concinnity in concision of the bacterial cell wall.
Dik et al. (2017)
9 more

4 mentions without citation

A Structural View at Vaccine Development against M. tuberculosis.
Romano et al. (2023)
3 more