› 4kn5

X-ray diffraction
1.7Å resolution

Crystal structure of a putative methylthioadenosine nucleosidase from Weissella paramesenteroides ATCC 33313 (Target NYSGRC-029342 )

Released:
DOI: 10.2210/pdb4kn5/pdb
PDB entry 4kn5 coloured by chain, front view.
PDB entry 4kn5 coloured by chain, side view.
PDB entry 4kn5 coloured by chain, top view.
Source organism: Weissella paramesenteroides ATCC 33313
Entry authors: Sampathkumar P, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
adenosylhomocysteine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 27.44 KDa
Source organism: Weissella paramesenteroides ATCC 33313
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5RAK4 (Residues: 2-231; Coverage: 100%)
Gene names: HMPREF0877_0999, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P43212
Unit cell:
a: 89.252Å b: 89.252Å c: 127.545Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.211
Expression system: Escherichia coli BL21(DE3)

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