› 4ksf

X-ray diffraction
3.1Å resolution

Crystal Structure of Malonyl-CoA decarboxylase from Agrobacterium vitis, Northeast Structural Genomics Consortium Target RiR35

Released:
DOI: 10.2210/pdb4ksf/pdb
PDB entry 4ksf coloured by chain, front view.
PDB entry 4ksf coloured by chain, side view.
PDB entry 4ksf coloured by chain, top view.
Source organism: Agrobacterium vitis S4
Primary publication:
Crystal structures of malonyl-coenzyme A decarboxylase provide insights into its catalytic mechanism and disease-causing mutations.
Froese DS, Forouhar F, Tran TH, Vollmar M, Kim YS, Lew S, Neely H, Seetharaman J, Shen Y, Xiao R, Acton TB, Everett JK, Cannone G, Puranik S, Savitsky P, Krojer T, Pilka ES, Kiyani W, Lee WH, Marsden BD, von Delft F, Allerston CK, Spagnolo L, Gileadi O, Montelione GT, Oppermann U, Yue WW, Tong L
OpenAccess logo Structure 21 1182-92 (2013)
PMID: 23791943

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110843 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malonyl-CoA decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 51.75 KDa
Source organism: Agrobacterium vitis S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9K0V9 (Residues: 1-449; Coverage: 97%)
Gene name: Avi_5372
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: I4122
Unit cell:
a: 100.412Å b: 100.412Å c: 242.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.22 0.291
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

From structural biology to designing therapy for inborn errors of metabolism.
Yue WW. (2016)
12 other articles

1 mention without citation

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