› 4kwk

X-ray diffraction
1.95Å resolution

Rat cysteine dioxygenase with cysteine persulfide bound to active site iron

Released:
DOI: 10.2210/pdb4kwk/pdb
PDB entry 4kwk coloured by chain, front view.
PDB entry 4kwk coloured by chain, side view.
PDB entry 4kwk coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Mechanistic implications of persulfenate and persulfide binding in the active site of cysteine dioxygenase.
Souness RJ, Kleffmann T, Tchesnokov EP, Wilbanks SM, Jameson GB, Jameson GN
Biochemistry 52 7606-17 (2013)
PMID: 24084026

Function and Biology Details

Reaction catalysed: 
L-cysteine + O(2) = 3-sulfinoalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 23.06 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21816 (Residues: 1-200; Coverage: 100%)
Gene name: Cdo1
Sequence domains: Cysteine dioxygenase type I
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand FE2 1 x FE2
Ligand CSS 1 x CSS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P43212
Unit cell:
a: 57.75Å b: 57.75Å c: 122.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
3 more