› 4kws

X-ray diffraction
1.64Å resolution

Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with Mg and glycerol

Released:
DOI: 10.2210/pdb4kws/pdb
PDB entry 4kws coloured by chain, front view.
PDB entry 4kws coloured by chain, side view.
PDB entry 4kws coloured by chain, top view.
Source organism: Chromohalobacter salexigens DSM 3043
Entry authors: Fedorov AA, Fedorov EV, Wichelecki D, Gerlt JA, Almo SC

Function and Biology Details

Reactions catalysed: 
D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactonate dehydratase family member ManD Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 405 amino acids
Theoretical weight: 45.47 KDa
Source organism: Chromohalobacter salexigens DSM 3043
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1QT89 (Residues: 2-403; Coverage: 100%)
Gene names: Csal_2974, manD
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 13 x MG
Ligand GOL 8 x GOL
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 195.273Å b: 85.762Å c: 195.097Å
α: 90° β: 110.31° γ: 90°
R-values:
R R work R free
0.14 0.138 0.162
Expression system: Escherichia coli

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