› 4l0e

X-ray diffraction
2.7Å resolution

Structure of P450sky (CYP163B3), a cytochrome P450 from skyllamycin biosynthesis (heme-coordinated expression tag)

Released:
DOI: 10.2210/pdb4l0e/pdb
PDB entry 4l0e coloured by chain, front view.
PDB entry 4l0e coloured by chain, side view.
PDB entry 4l0e coloured by chain, top view.
Source organism: Streptomyces sp. Acta 2897
Primary publication:
Cytochrome p450sky interacts directly with the nonribosomal peptide synthetase to generate three amino acid precursors in skyllamycin biosynthesis.
Uhlmann S, Süssmuth RD, Cryle MJ
ACS Chem Biol 8 2586-96 (2013)
PMID: 24079328

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p450 monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 446 amino acids
Theoretical weight: 49.37 KDa
Source organism: Streptomyces sp. Acta 2897
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F2YRY7 (Residues: 1-423; Coverage: 100%)
Gene name: sky32
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 91.62Å b: 91.62Å c: 123.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.248 0.293
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Nonribosomal Peptide Synthesis-Principles and Prospects.
Süssmuth et al. (2017)
9 more

2 mentions without citation

Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.
Rudolf et al. (2017)
1 more