› 4l0l

X-ray diffraction
2.1Å resolution

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-560256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan D,D-transpeptidase FtsI Chain: A
Molecule details ›
Chain: A
Length: 537 amino acids
Theoretical weight: 58.19 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51504 (Residues: 50-579; Coverage: 92%)
Gene names: ALP65_00912, CAZ10_21230, GNQ48_11285, GUL26_13375, IPC1295_15535, IPC737_20650, L4V69_30060, PAERUG_P19_London_7_VIM_2_05_10_00246, ftsI, ftsI_1, pbpB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PFV 1 x PFV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 67.725Å b: 82.832Å c: 88.689Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.275
Expression system: Escherichia coli

Quick links

Citations

8 review citations

New β-Lactamase Inhibitors in the Clinic.
Papp-Wallace et al. (2016)
7 more

1 mention without citation

The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes.
Cleverley et al. (2019)