› 4l5y

X-ray diffraction
2.1Å resolution

Methylthioadenosine phosphorylase from Schistosoma mansoni in APO form

Released:
DOI: 10.2210/pdb4l5y/pdb
PDB entry 4l5y coloured by chain, front view.
PDB entry 4l5y coloured by chain, side view.
PDB entry 4l5y coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
Crystal Structure of Schistosoma mansoni Adenosine Phosphorylase/5'-Methylthioadenosine Phosphorylase and Its Importance on Adenosine Salvage Pathway.
Torini JR, Brandão-Neto J, DeMarco R, Pereira HD
OpenAccess logo PLoS Negl Trop Dis 10 e0005178 (2016)
PMID: 27935959

Function and Biology Details

Reaction catalysed: 
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-methyl-5'-thioadenosine phosphorylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 320 amino acids
Theoretical weight: 35.26 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I0B503 (Residues: 1-299; Coverage: 100%)
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P21
Unit cell:
a: 80.626Å b: 82.4Å c: 150.286Å
α: 90° β: 101.6° γ: 90°
R-values:
R R work R free
0.186 0.184 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Pyrimidine metabolism in schistosomes: A comparison with other parasites and the search for potential chemotherapeutic targets.
El Kouni MH. (2017)
3 other articles

1 mention without citation

Crystal Structure of Schistosoma mansoni Adenosine Phosphorylase/5'-Methylthioadenosine Phosphorylase and Its Importance on Adenosine Salvage Pathway.
Torini et al. (2016)